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ptpn13 rna  (Radboud University)

 
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    Structured Review

    Radboud University ptpn13 rna
    (a) Protein structures are shown encoded by ptpn20 homologue and the immediately 5′ upstream FRMPD2 , as currently annotated in five fish genomes, the human genome and the mouse genome. In some cases like Fugu and Tetraodon a single known coding transcript exists besides separate transcripts encoding the PTP domain and the “FRMPDptpn13 (PTPBL) is added below. (b) Primers were designed as indicated, leaving approximately 100 bp known coding sequence for the purpose of alignment of generated sequences. PCR products with forward primers on the second to last known exon of human and zebrafish FRMPD2 and reverse oligos on the second exon of PTPN20 . A schematic representation of retrieved sequences blasted to the genome are indicated in green (not to scale). (c) Generated PCR products on human (top) and zebrafish (bottom) cDNA libraries using the described primer sets. Generated band sizes are consistent with expected values based on homology with the ptpn13 gene. " width="250" height="auto" />
    Ptpn13 Rna, supplied by Radboud University, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptpn13 rna/product/Radboud University
    Average 90 stars, based on 1 article reviews
    ptpn13 rna - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA"

    Article Title: Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0035913

    (a) Protein structures are shown encoded by ptpn20 homologue and the immediately 5′ upstream FRMPD2 , as currently annotated in five fish genomes, the human genome and the mouse genome. In some cases like Fugu and Tetraodon a single known coding transcript exists besides separate transcripts encoding the PTP domain and the “FRMPD
    Figure Legend Snippet: (a) Protein structures are shown encoded by ptpn20 homologue and the immediately 5′ upstream FRMPD2 , as currently annotated in five fish genomes, the human genome and the mouse genome. In some cases like Fugu and Tetraodon a single known coding transcript exists besides separate transcripts encoding the PTP domain and the “FRMPD" part. For comparison the protein structure encoded by human ptpn13 (PTPBL) is added below. (b) Primers were designed as indicated, leaving approximately 100 bp known coding sequence for the purpose of alignment of generated sequences. PCR products with forward primers on the second to last known exon of human and zebrafish FRMPD2 and reverse oligos on the second exon of PTPN20 . A schematic representation of retrieved sequences blasted to the genome are indicated in green (not to scale). (c) Generated PCR products on human (top) and zebrafish (bottom) cDNA libraries using the described primer sets. Generated band sizes are consistent with expected values based on homology with the ptpn13 gene.

    Techniques Used: Comparison, Sequencing, Generated

    Morpholinos targeting ptpn13 and ptpn20 were injected in the zebrafish at the one cell stage, and concentrations were titrated down until no phenotype was observed. Normal (red), low (green) concentrations and combined low concentrations of ptpn13 and ptpn20 morpholino were micro-injected and embryos were grown to 3dpf under normal conditions. Pictures were taken from all embryos and tails were measured using ImageJ imaging software, from the yolk to the tip of the tail, and compared to non-injected control. Average tail length compared to non-injected control is plotted as a percentage deviating from 100% in (a) and representative fish are shown for each condition in (b). Zebrafish embryos were microinjected as described above, using low concentration combined knockdown of ptpra with either ptpn13 or ptpn20 , or ptpre with either ptpn13 or ptpn20 and tail lengths are plotted in (c) and (d). (e) Shown are representative fish from the experiments depicted in (c) and (d). All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001. Morpholino concentrations are color coded: red for “full
    Figure Legend Snippet: Morpholinos targeting ptpn13 and ptpn20 were injected in the zebrafish at the one cell stage, and concentrations were titrated down until no phenotype was observed. Normal (red), low (green) concentrations and combined low concentrations of ptpn13 and ptpn20 morpholino were micro-injected and embryos were grown to 3dpf under normal conditions. Pictures were taken from all embryos and tails were measured using ImageJ imaging software, from the yolk to the tip of the tail, and compared to non-injected control. Average tail length compared to non-injected control is plotted as a percentage deviating from 100% in (a) and representative fish are shown for each condition in (b). Zebrafish embryos were microinjected as described above, using low concentration combined knockdown of ptpra with either ptpn13 or ptpn20 , or ptpre with either ptpn13 or ptpn20 and tail lengths are plotted in (c) and (d). (e) Shown are representative fish from the experiments depicted in (c) and (d). All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001. Morpholino concentrations are color coded: red for “full" knockdown, giving full phenotype without being toxic and green for “low" concentration, giving no observable phenotype.

    Techniques Used: Injection, Imaging, Software, Control, Concentration Assay, Knockdown

    (a) Low dose combined knockdowns of ptpn13 or ptpn20 and arhgap29b were performed by injecting indicated amounts of morpholino at the one cell stage. Tail lengths were measured at 3dpf and plotted. Co-knockdowns with arhgap5 were included as a control. (b) Similar co-knockdowns as in (a) but with ptpra and ptpre knockdown instead of ptpn13 and ptpn20 knockdown. (c) Zebrafish embryos were micro-injected with morpholinos targeting the different phosphatases in low concentrations together with low dose arhgef27 ( ngef ) morpholino. Embryos were grown to 3 dpf and tail lengths were determined and plotted as a percentage of non-injected control. All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001.
    Figure Legend Snippet: (a) Low dose combined knockdowns of ptpn13 or ptpn20 and arhgap29b were performed by injecting indicated amounts of morpholino at the one cell stage. Tail lengths were measured at 3dpf and plotted. Co-knockdowns with arhgap5 were included as a control. (b) Similar co-knockdowns as in (a) but with ptpra and ptpre knockdown instead of ptpn13 and ptpn20 knockdown. (c) Zebrafish embryos were micro-injected with morpholinos targeting the different phosphatases in low concentrations together with low dose arhgef27 ( ngef ) morpholino. Embryos were grown to 3 dpf and tail lengths were determined and plotted as a percentage of non-injected control. All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001.

    Techniques Used: Control, Knockdown, Injection



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    ptpn13 rna  (Radboud University)
    90
      Buy from Supplier

    Image Search Results


    (a) Protein structures are shown encoded by ptpn20 homologue and the immediately 5′ upstream FRMPD2 , as currently annotated in five fish genomes, the human genome and the mouse genome. In some cases like Fugu and Tetraodon a single known coding transcript exists besides separate transcripts encoding the PTP domain and the “FRMPD

    Journal: PLoS ONE

    Article Title: Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA

    doi: 10.1371/journal.pone.0035913

    Figure Lengend Snippet: (a) Protein structures are shown encoded by ptpn20 homologue and the immediately 5′ upstream FRMPD2 , as currently annotated in five fish genomes, the human genome and the mouse genome. In some cases like Fugu and Tetraodon a single known coding transcript exists besides separate transcripts encoding the PTP domain and the “FRMPD" part. For comparison the protein structure encoded by human ptpn13 (PTPBL) is added below. (b) Primers were designed as indicated, leaving approximately 100 bp known coding sequence for the purpose of alignment of generated sequences. PCR products with forward primers on the second to last known exon of human and zebrafish FRMPD2 and reverse oligos on the second exon of PTPN20 . A schematic representation of retrieved sequences blasted to the genome are indicated in green (not to scale). (c) Generated PCR products on human (top) and zebrafish (bottom) cDNA libraries using the described primer sets. Generated band sizes are consistent with expected values based on homology with the ptpn13 gene.

    Article Snippet: Ptpn13 RNA was transcribed from full length mouse cDNA kindly provided by Wiljan Hendriks (Department of Cell Biology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands).

    Techniques: Comparison, Sequencing, Generated

    Morpholinos targeting ptpn13 and ptpn20 were injected in the zebrafish at the one cell stage, and concentrations were titrated down until no phenotype was observed. Normal (red), low (green) concentrations and combined low concentrations of ptpn13 and ptpn20 morpholino were micro-injected and embryos were grown to 3dpf under normal conditions. Pictures were taken from all embryos and tails were measured using ImageJ imaging software, from the yolk to the tip of the tail, and compared to non-injected control. Average tail length compared to non-injected control is plotted as a percentage deviating from 100% in (a) and representative fish are shown for each condition in (b). Zebrafish embryos were microinjected as described above, using low concentration combined knockdown of ptpra with either ptpn13 or ptpn20 , or ptpre with either ptpn13 or ptpn20 and tail lengths are plotted in (c) and (d). (e) Shown are representative fish from the experiments depicted in (c) and (d). All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001. Morpholino concentrations are color coded: red for “full

    Journal: PLoS ONE

    Article Title: Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA

    doi: 10.1371/journal.pone.0035913

    Figure Lengend Snippet: Morpholinos targeting ptpn13 and ptpn20 were injected in the zebrafish at the one cell stage, and concentrations were titrated down until no phenotype was observed. Normal (red), low (green) concentrations and combined low concentrations of ptpn13 and ptpn20 morpholino were micro-injected and embryos were grown to 3dpf under normal conditions. Pictures were taken from all embryos and tails were measured using ImageJ imaging software, from the yolk to the tip of the tail, and compared to non-injected control. Average tail length compared to non-injected control is plotted as a percentage deviating from 100% in (a) and representative fish are shown for each condition in (b). Zebrafish embryos were microinjected as described above, using low concentration combined knockdown of ptpra with either ptpn13 or ptpn20 , or ptpre with either ptpn13 or ptpn20 and tail lengths are plotted in (c) and (d). (e) Shown are representative fish from the experiments depicted in (c) and (d). All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001. Morpholino concentrations are color coded: red for “full" knockdown, giving full phenotype without being toxic and green for “low" concentration, giving no observable phenotype.

    Article Snippet: Ptpn13 RNA was transcribed from full length mouse cDNA kindly provided by Wiljan Hendriks (Department of Cell Biology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands).

    Techniques: Injection, Imaging, Software, Control, Concentration Assay, Knockdown

    (a) Low dose combined knockdowns of ptpn13 or ptpn20 and arhgap29b were performed by injecting indicated amounts of morpholino at the one cell stage. Tail lengths were measured at 3dpf and plotted. Co-knockdowns with arhgap5 were included as a control. (b) Similar co-knockdowns as in (a) but with ptpra and ptpre knockdown instead of ptpn13 and ptpn20 knockdown. (c) Zebrafish embryos were micro-injected with morpholinos targeting the different phosphatases in low concentrations together with low dose arhgef27 ( ngef ) morpholino. Embryos were grown to 3 dpf and tail lengths were determined and plotted as a percentage of non-injected control. All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001.

    Journal: PLoS ONE

    Article Title: Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA

    doi: 10.1371/journal.pone.0035913

    Figure Lengend Snippet: (a) Low dose combined knockdowns of ptpn13 or ptpn20 and arhgap29b were performed by injecting indicated amounts of morpholino at the one cell stage. Tail lengths were measured at 3dpf and plotted. Co-knockdowns with arhgap5 were included as a control. (b) Similar co-knockdowns as in (a) but with ptpra and ptpre knockdown instead of ptpn13 and ptpn20 knockdown. (c) Zebrafish embryos were micro-injected with morpholinos targeting the different phosphatases in low concentrations together with low dose arhgef27 ( ngef ) morpholino. Embryos were grown to 3 dpf and tail lengths were determined and plotted as a percentage of non-injected control. All error bars are standard error of the mean. Student t-test was performed where indicated; no asterisk indicates P>0.05, * indicates 0.05>P>0.001 and ** indicates P<0.001.

    Article Snippet: Ptpn13 RNA was transcribed from full length mouse cDNA kindly provided by Wiljan Hendriks (Department of Cell Biology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands).

    Techniques: Control, Knockdown, Injection